Species pattern and genetic diversity of Trichoderma in a mid-European, primeval floodplain-forest.
Identifieur interne : 004380 ( Main/Exploration ); précédent : 004379; suivant : 004381Species pattern and genetic diversity of Trichoderma in a mid-European, primeval floodplain-forest.
Auteurs : Michael Wuczkowski [Autriche] ; Irina Druzhinina ; Youssuf Gherbawy ; Brigitte Klug ; Hansjörg Prillinger ; Christian P. KubicekSource :
- Microbiological research [ 0944-5013 ] ; 2003.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Arbres (microbiologie), Autriche (MeSH), Espaceur de l'ADN ribosomique (génétique), Gènes d'ARN ribosomique (MeSH), Microbiologie du sol (MeSH), Phylogenèse (MeSH), Populus (microbiologie), Racines de plante (microbiologie), Réaction de polymérisation en chaîne (MeSH), Salix (microbiologie), Technique RAPD (MeSH), Techniques de typage mycologique (MeSH), Trichoderma (classification), Trichoderma (cytologie), Trichoderma (génétique), Trichoderma (isolement et purification), Variation génétique (MeSH).
- MESH :
- cytologie : Trichoderma.
- génétique : Espaceur de l'ADN ribosomique, Trichoderma.
- isolement et purification : Trichoderma.
- microbiologie : Arbres, Populus, Racines de plante, Salix.
- Analyse de séquence d'ADN, Autriche, Gènes d'ARN ribosomique, Microbiologie du sol, Phylogenèse, Réaction de polymérisation en chaîne, Technique RAPD, Techniques de typage mycologique, Variation génétique.
- Wicri :
- geographic : Autriche.
English descriptors
- KwdEn :
- Austria (MeSH), DNA, Ribosomal Spacer (genetics), Genes, rRNA (MeSH), Genetic Variation (MeSH), Mycological Typing Techniques (MeSH), Phylogeny (MeSH), Plant Roots (microbiology), Polymerase Chain Reaction (MeSH), Populus (microbiology), Random Amplified Polymorphic DNA Technique (MeSH), Salix (microbiology), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH), Trees (microbiology), Trichoderma (classification), Trichoderma (cytology), Trichoderma (genetics), Trichoderma (isolation & purification).
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- geographic : Austria.
- classification : Trichoderma.
- cytology : Trichoderma.
- genetics : Trichoderma.
- isolation & purification : Trichoderma.
- microbiology : Plant Roots, Populus, Salix, Trees.
- Genes, rRNA, Genetic Variation, Mycological Typing Techniques, Phylogeny, Polymerase Chain Reaction, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Soil Microbiology.
Abstract
We investigated the occurrence and genetic diversity of Trichoderma in the river Danube national park, a primeval, riparian forest area located south-east of Vienna (Austria) which represents one of the last cases of an original European river-floodplain landscape. Forty-six strains were isolated and identified at the species level by analysis of morphological characters, by sequence analysis of their internal transcribed spacer regions 1 and 2 (ITS 1 and 2) of the rDNA cluster and--in some cases--a fragment of the translation elongation factor 1alpha (tef1) gene, and RAPD-analysis. Twenty-one strains were positively identified as T. harzianum, thirteen as T. rossicum, four as T. cerinum, two as T. hamatum, and one each as T. atroviride and T. koningii: four strains yielded two different ITS1 and 2 as well as tef1 sequence types, which were not alignable with any known species. Our studies show that they represent two new taxa of Trichoderma.
DOI: 10.1078/0944-5013-00193
PubMed: 12906385
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Forty-six strains were isolated and identified at the species level by analysis of morphological characters, by sequence analysis of their internal transcribed spacer regions 1 and 2 (ITS 1 and 2) of the rDNA cluster and--in some cases--a fragment of the translation elongation factor 1alpha (tef1) gene, and RAPD-analysis. Twenty-one strains were positively identified as T. harzianum, thirteen as T. rossicum, four as T. cerinum, two as T. hamatum, and one each as T. atroviride and T. koningii: four strains yielded two different ITS1 and 2 as well as tef1 sequence types, which were not alignable with any known species. Our studies show that they represent two new taxa of Trichoderma.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12906385</PMID><DateCompleted><Year>2003</Year><Month>09</Month><Day>12</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0944-5013</ISSN><JournalIssue CitedMedium="Print"><Volume>158</Volume><Issue>2</Issue><PubDate><Year>2003</Year></PubDate></JournalIssue><Title>Microbiological research</Title><ISOAbbreviation>Microbiol Res</ISOAbbreviation></Journal><ArticleTitle>Species pattern and genetic diversity of Trichoderma in a mid-European, primeval floodplain-forest.</ArticleTitle><Pagination><MedlinePgn>125-33</MedlinePgn></Pagination><Abstract><AbstractText>We investigated the occurrence and genetic diversity of Trichoderma in the river Danube national park, a primeval, riparian forest area located south-east of Vienna (Austria) which represents one of the last cases of an original European river-floodplain landscape. Forty-six strains were isolated and identified at the species level by analysis of morphological characters, by sequence analysis of their internal transcribed spacer regions 1 and 2 (ITS 1 and 2) of the rDNA cluster and--in some cases--a fragment of the translation elongation factor 1alpha (tef1) gene, and RAPD-analysis. Twenty-one strains were positively identified as T. harzianum, thirteen as T. rossicum, four as T. cerinum, two as T. hamatum, and one each as T. atroviride and T. koningii: four strains yielded two different ITS1 and 2 as well as tef1 sequence types, which were not alignable with any known species. Our studies show that they represent two new taxa of Trichoderma.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wuczkowski</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Austrian Centre of Biological Resources and Applied Mycology, Institute of Applied Microbiology, University of Agricultural Sciences, Nussdorfer Lände 11, A-1190 Wien, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Druzhinina</LastName><ForeName>Irina</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Gherbawy</LastName><ForeName>Youssuf</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Klug</LastName><ForeName>Brigitte</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Prillinger</LastName><ForeName>Hansjörg</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Kubicek</LastName><ForeName>Christian 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purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>8</Month><Day>9</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>9</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>8</Month><Day>9</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12906385</ArticleId><ArticleId IdType="pii">S0944-5013(04)70111-1</ArticleId><ArticleId IdType="doi">10.1078/0944-5013-00193</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Autriche</li></country></list><tree><noCountry><name sortKey="Druzhinina, Irina" sort="Druzhinina, Irina" uniqKey="Druzhinina I" first="Irina" last="Druzhinina">Irina Druzhinina</name><name sortKey="Gherbawy, Youssuf" sort="Gherbawy, Youssuf" uniqKey="Gherbawy Y" first="Youssuf" last="Gherbawy">Youssuf Gherbawy</name><name sortKey="Klug, Brigitte" sort="Klug, Brigitte" uniqKey="Klug B" first="Brigitte" last="Klug">Brigitte Klug</name><name sortKey="Kubicek, Christian P" sort="Kubicek, Christian P" uniqKey="Kubicek C" first="Christian P" last="Kubicek">Christian P. Kubicek</name><name sortKey="Prillinger, Hansjorg" sort="Prillinger, Hansjorg" uniqKey="Prillinger H" first="Hansjörg" last="Prillinger">Hansjörg Prillinger</name></noCountry><country name="Autriche"><noRegion><name sortKey="Wuczkowski, Michael" sort="Wuczkowski, Michael" uniqKey="Wuczkowski M" first="Michael" last="Wuczkowski">Michael Wuczkowski</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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